Caspase 9 Activity Assay Kit (Colorimetric Method)

• Detection Method:

  Colorimetric method

• Assay Principle:

  Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.

• Sample Type:

  Cell and tissue samples

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0141813025006841

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0925521423003162

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0925521423001369

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0925521422002678

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0925521424005106

• Citation 06:

  https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c09673

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S1773224724008281

• Citation 08:

  https://link.springer.com/article/10.1007/s12035-025-04810-y

• Citation 09:

  https://www.nature.com/articles/s41598-025-96788-9

• Citation 10:

  https://link.springer.com/article/10.1007/s00210-025-04355-w

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S1756464624001713

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S0039128X22001908

• Detection Instrument:

  Spectrophotometer; Microplate reader