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Caspase 2 Activity Assay Kit (Colorimetric Method)

CAT: 0763-E-CK-A382-01Size: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A382-01Size:20 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Colorimetric method
Assay Principle
Caspase 2, also known as Ich-1 (ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis. Caspase 2 can be activated by caspase 1, caspase 3 and granzyme B in vitro. This caspase 2 activity assay kit is based on caspase 2 that can catalyze the substrate Ac-VDQQD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 2 can be calculated by determining the absorbance at 405 nm.
Sample Type
Cell and tissue samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Detection Instrument
Spectrophotometer; Microplate reader

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