Caspase 2 Activity Assay Kit (Colorimetric Method)

CAT:
763-E-CK-A382-01
Size:
20 Assays

For Laboratory Research Only. Not for Clinical or Personal Use.

  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Caspase 2 Activity Assay Kit (Colorimetric Method) - image 1

Caspase 2 Activity Assay Kit (Colorimetric Method)

  • Type:

    Apoptosis / Caspase
  • Detection Method:

    Colorimetric method
  • Assay Principle:

    Caspase 2, also known as Ich-1 (ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis. Caspase 2 can be activated by caspase 1, caspase 3 and granzyme B in vitro. This caspase 2 activity assay kit is based on caspase 2 that can catalyze the substrate Ac-VDQQD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 2 can be calculated by determining the absorbance at 405 nm.
  • Assay Performance Time:

    4 - 6 Hours
  • Sample Type:

    Cell and tissue samples
  • Shipping Conditions:

    Ice Bag
  • Storage Conditions:

    This product can be stored at -20°C for 12 months with shading light.
  • Detection Instrument:

    Spectrophotometer
  • Result Type:

    Qualitative or quantitative