Caspase 8 Activity Assay Kit (Colorimetric Method)

• Type:

  Apoptosis / Caspase

• Detection Method:

  Colorimetric method

• Assay Principle:

  Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.

• Assay Performance Time:

  4 - 6 Hours

• Sample Type:

  Cell and tissue samples

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.frontiersin.org/articles/10.3389/fimmu.2021.748758/full

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/s0925521423001369

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/s0925521423003162

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/s0925521422002678

• Detection Instrument:

  Spectrophotometer

• Result Type:

  Qualitative or quantitative