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Caspase 8 Activity Assay Kit (Colorimetric Method)

CAT: 0763-E-CK-A388-01Size: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A388-01Size:20 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Colorimetric method
Assay Principle
Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.
Sample Type
Cell and tissue samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0141813025014394
Citation 02
https://www.frontiersin.org/articles/10.3389/fimmu.2021.748758/full
Citation 03
https://www.sciencedirect.com/science/article/pii/S0925521423001369
Citation 04
https://www.sciencedirect.com/science/article/pii/S0925521423003162
Citation 05
https://www.sciencedirect.com/science/article/pii/S0925521422002678
Citation 06
https://www.sciencedirect.com/science/article/pii/S0925521424005106
Citation 07
https://pmc.ncbi.nlm.nih.gov/articles/PMC11989111/
Citation 08
https://link.springer.com/article/10.1007/s12035-025-04810-y
Detection Instrument
Spectrophotometer; Microplate reader

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