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Caspase 6 Activity Assay Kit (Colorimetric Method)

CAT: 0763-E-CK-A386-01Size: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A386-01Size:20 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Colorimetric method
Assay Principle
Caspase 6, also known as Mch-2, was originally discovered in human Jurkat cells. The precursor of caspase 6 can be cleaved by granzyme B to form an activated caspase 6 dimer, which is found to induce apoptosis. Caspase 6 can cleave PARP and keratin-18, and also cleave Lamin A, a key component protein on the nuclear envelope. Only caspase 6 can cleave Lamin A in the caspase family. This caspase 6 activity assay kit is based on caspase 6 that can catalyze the substrate Ac-VEID-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 6 can be calculated by determining the absorbance at 405 nm.
Sample Type
Cell and tissue samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012737
Detection Instrument
Spectrophotometer; Microplate reader

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