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Caspase 3/7 Activity Assay Kit (Colorimetric Method)

CAT: 0763-E-CK-A383-01Size: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A383-01Size:20 Assays
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Colorimetric method
Assay Principle
Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
Sample Type
Cell and tissue samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://pubs.acs.org/doi/abs/10.1021/acs.est.4c10984
Citation 02
https://www.tandfonline.com/doi/abs/10.2147/IJN.S417928
Citation 03
https://www.sciencedirect.com/science/article/pii/S0925521423003162
Citation 04
https://www.sciencedirect.com/science/article/pii/S0925521423001369
Citation 05
https://www.sciencedirect.com/science/article/pii/S0141813025014394
Citation 06
https://www.sciencedirect.com/science/article/pii/S0141813025006841
Citation 07
https://www.sciencedirect.com/science/article/pii/S0141813024082667
Citation 08
https://www.sciencedirect.com/science/article/pii/S0141813025035822
Citation 09
https://www.sciencedirect.com/science/article/pii/S0141813025042424
Citation 10
https://www.mdpi.com/2073-4409/12/23/2734
Citation 11
https://www.mdpi.com/2072-6643/14/21/4605
Citation 12
https://www.sciencedirect.com/science/article/pii/S0925521422002678
Detection Instrument
Spectrophotometer; Microplate reader

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