Caspase 1 Activity Assay Kit (Colorimetric Method)

• Type:

  Apoptosis / Caspase

• Detection Method:

  Colorimetric method

• Assay Principle:

  Caspase 1, also known as Interkeukin 1β Conberting Enzyme (ICE), is the only caspase in the caspase family that can cleave IL-1β prefixes or IL-18 prefabrication. Caspase 11 can cleave 45 kD caspase 1 preparation to produce 20 kD and 10 kD fragments, which can form heterodimer and further form tetramer by two heterodimers. Caspase 1 regulates apoptosis by cleaving Bcl-XL and the associated immune response by cleaving cytokine precursors, or regulates pyroptosis by hydrolyzing Gastermin D (GSDMD) . This caspase 1 activity assay kit is based on caspase 1 that can catalyze the substrate AC-YVAD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 1 can be calculated by determining the absorbance at 405 nm.

• Assay Performance Time:

  4 - 6 Hours

• Sample Type:

  Cell and tissue samples

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/s0024320524005563

• Detection Instrument:

  Spectrophotometer

• Result Type:

  Qualitative or quantitative