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Human IL-33 Protein

CAT: 0749-PRP1018-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1018-01Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researchers suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also plays a pivotal role in helminthic infection and allergic disorders.
Description
Human IL-33 protein, expressed in E. coli
Product Name Alternative
C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; Interleukin-33; IL33
Gene ID
90865
Accession Number
O95760
Tag
IL-33
Type
Cytokines and proteins
Applications
No tag
Purity
> 98 % as determined by SDS-PAGE
Activity
Testing in progress
Molecular Weight
18.1 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human IL-33 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant human IL-33 consists of 160 amino acids (N-Met) and has a predicted molecular mass of 18.1 kDa. The apparent molecular mass of the human IL-33 is approximately 18 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20 mM Tris,50 mM NaCl, pH 5.0,1 mM DTT and 1 mM EDTA.
Applications Notes
Human
Other Product Names
C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; Interleukin-33; IL33
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL33 protein protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/O95760
AA Sequence
Amino acid sequence derived from human IL-33 isoform (O95760) (Ser112-Thr270) was expressed.

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