Cullin 2 Recombinant Rabbit Monoclonal Antibody [JG72-31]

Cullin 2 Recombinant Rabbit Monoclonal Antibody [JG72-31]

• Description: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex.
• Product Name Alternative: Cul2 antibody CUL 2 antibody CUL-2 antibody CUL2 antibody CUL2_HUMAN antibody cullin 2 antibody cullin homolog 2 antibody Cullin-2 antibody MGC131970 antibody
• Abbreviation: CUL-2, CUL2
• CAS Number: 9000-83-3
• Swiss Prot: Q13617 Human, Q9D4H8 Mouse,
• Cellular Locus: Cytoplasm. Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within N-terminal Human Cullin 2 .
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IP
• Positive Control: PC-3M cell lysate, k562 tissue lysate, rat testis tissue, human tonsil tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 89 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes