Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE57-80]

Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE57-80]

• Description: This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR (KLHL42) is involved in ubiquitination of KATNA1.
• Product Name Alternative: CUL 3 antibody Cul-3 antibody cul3 antibody CUL3_HUMAN antibody Cullin 3 antibody Cullin-3 antibody Cullin3 antibody KIAA0617 antibody PHA2E antibody
• Abbreviation: Cullin-3 CUL-3 CUL3 KIAA0617
• CAS Number: 9000-83-3
• Swiss Prot: Q13618 Human, Q9JLV5 Mouse, B5DF89 Rat
• Cellular Locus: Nucleus, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human Cullin 3 aa 719-768 / 768.
• Isotype: IgG
• Clone: JE57-80
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: HeLa cell lysate, Jurkat cell lysate, PC-12 cell lysate, mouse spleen tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, rat hippocampus tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:500-1:2,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 89 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes