Cullin 4a Recombinant Rabbit Monoclonal Antibody [JU07-33]

Cullin 4a Recombinant Rabbit Monoclonal Antibody [JU07-33]

• Description: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX (DET1-COP1) directs ubiquitination of JUN. DCX (DDB2) directs ubiquitination of XPC. DCX (DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.
• Product Name Alternative: 2810470J21Rik antibody AW495282 antibody CUL 4A antibody CUL-4A antibody Cul4a antibody Cul4a protein antibody CUL4A_HUMAN antibody Cullin-4A antibody MGC36573 antibody MGC64071 antibody
• Abbreviation: CUL-4A, CUL4A
• CAS Number: 9000-83-3
• Swiss Prot: Q13619 Human
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within Human Cullin 4a aa 710-759 / 759.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC
• Positive Control: Hela cell lysate, SiHa cell lysate, A431, HUVEC, human liver carcinoma tissue, human cervix tissue, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, Hela.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 88 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes