GFP Recombinant Rabbit Monoclonal Antibody [SY0243]

GFP Recombinant Rabbit Monoclonal Antibody [SY0243]

• Description: The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
• Product Name Alternative: GFP antibody Green fluorescent protein antibody yfp antibody
• CAS Number: 9000-83-3
• Swiss Prot: P42212 AequoreaVictoria
• Host: Rabbit
• Species Reactivity: Universal
• Immunogen: Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IP, IHC-P
• Positive Control: GFP recombinant protein.
• Concentration: 1 mg/mL
• Dilution: WB: 1:10,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IP: 2-5 µg/ml. ;IHC-P: 1:5,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 27 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes