Mouse anti Human Bence Jones lambda (surface and hidden determinants), conjugated with Biotin

• Background:

  The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

• CAS Number:

  9007-83-4

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  NI 412, NI 268

• Conjugation:

  Biotin

• Type:

  Monoclonal Antibody

• Source:

  Highly purified Bence Jones lambda proteins isolated from pooled Human urine.

• Purification Method:

  ProteinA/G affinity purification

• Assay Principle:

  ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

• Form:

  No preservative added, as it may interfere with the antibody activity; Noforeign protein added

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Shipping Conditions:

  Shipped lyophilized at ambient temperature

• Storage Conditions:

  The lyophilized product may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day