Mouse anti Human CD54

Mouse anti Human CD54

• Background: CD54, M.W. 90,000, antigen is the Intercellular Adhesion Molecule-1 (ICAM-1) and is the ligand for LFA-1 mediated adhesion. CD54 is weakly expressed on peripheral blood leukocytes. Upon activation the antigen is expressed strongly on B cells, T cells, macrophages and granulocytes. ICAM-1 is also inducible on endothelial cells, epithelial cells and fibroblasts.
• Synonyms: CD54
• CAS Number: 9007-83-4
• UniProt: P05362
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG2b
• Clone: D3.6
• Conjugation: Unconjugated
• Type: Single-Color Reagent
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20º C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. ICAM-1 a ligand for LFA-1 dependent adhesion of B,T and myeloid cells. Makgoba, M.W., Sanders, M.E., Ginther, Luce, G.E., Dustin, M.L., Springer,T.A., Clark, E.A., Mannoni, P., Shaw, S., Nature, 1988:331:86. 2. Supergene families meet in the immune system. Dustin, M.L., Staunton, D.E., and Springer, T.A., Immunol. Today. 1988:9:213. 3. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunto, D.E., Dustin, M.L., and Springer, T.A., Nature. 1989:339:61. 4. Leukocytes adhesion to cells. Molecular basis, Physiological Relevance and Abnormalities. Patarroyo, M., and Makgoba, M.W., Scand. J. Immunol. 1989;30:129. 5. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber, W., and Merluzzi, V.J., Nature. 1990;44:70.
• Shipping Conditions: Room Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: Flow Cytometry Staining