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Mouse anti Human CD54

CAT: 0579-0541Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0579-0541Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CD54, M.W. 90,000, antigen is the Intercellular Adhesion Molecule-1 (ICAM-1) and is the ligand for LFA-1 mediated adhesion. CD54 is weakly expressed on peripheral blood leukocytes. Upon activation the antigen is expressed strongly on B cells, T cells, macrophages and granulocytes. ICAM-1 is also inducible on endothelial cells, epithelial cells and fibroblasts.
CAS Number
9007-83-4
Synonyms
CD54
UniProt
P05362
Host
Mouse
Species Reactivity
Human
Isotype
IgG2b
Clone
D3.6
Conjugation
Unconjugated
Type
Single-Color Reagent
Applications
Flow Cytometry
Field of Research
Flow Cytometry
Purification Method
Protein A/G Chromatography
Assay Principle
PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20º C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. ICAM-1 a ligand for LFA-1 dependent adhesion of B,T and myeloid cells. Makgoba, M.W., Sanders, M.E., Ginther, Luce, G.E., Dustin, M.L., Springer,T.A., Clark, E.A., Mannoni, P., Shaw, S., Nature, 1988:331:86. 2. Supergene families meet in the immune system. Dustin, M.L., Staunton, D.E., and Springer, T.A., Immunol. Today. 1988:9:213. 3. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunto, D.E., Dustin, M.L., and Springer, T.A., Nature. 1989:339:61. 4. Leukocytes adhesion to cells. Molecular basis, Physiological Relevance and Abnormalities. Patarroyo, M., and Makgoba, M.W., Scand. J. Immunol. 1989;30:129. 5. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber, W., and Merluzzi, V.J., Nature. 1990;44:70.
Shipping Conditions
Room Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
Flow Cytometry Staining

UniProtKB · P05362

Intercellular adhesion molecule 1

ICAM1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05362
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ICAM1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ICAM-1
EC number
—
Processing
Precursor
Secondary accessions
B2R6M3, Q5NKV7, Q96B50
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesionHost-virus interaction

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Molecular function

Host cell receptor for virus entryReceptor

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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