Mouse anti Human CD25

• Background:

  Mouse anti Human CD25 can be used for the identification of Human receptor for Interleukin-2 (IL-2R) expressing the 55 kDa surface antigen.

• Synonyms:

  CD25

• UniProt:

  P01589

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  ITYV

• Conjugation:

  Unconjugated

• Type:

  Single-Color Reagent

• Source:

  Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated Human T-lymphocytes.

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. _x000D_ WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. A Monoclonal Antibody (Anti-Tac) Reactive with Activated and Functionally Mature Human T Cells. Uchiyama T., Broder S., Waldmann TA, 1981 J. Immunol.126,1393. _x000D_ 2. Direct Demonstration of the Identity of T Cell Growth Factor Binding Protein and the Tac Antigen. Robb RJ, Greene WC, J. Exp. Med. 1983,158;1332. _x000D_ 3. Patients with HIV infection have a reduced proportion of lymphocytes expressing the IL2 receptor p55 chain (TAC, CD25). Zola H., Koh L.Y., Mantzioris B.X., Rhodes D., Clin. Immunol. Immunopathol. 1991 Ap:59(1):16-25. _x000D_ 4. Spontaneous lymphocyte proliferation in HTLV-I/II infection reflects preferential activation of CD8 and CD16/56 cell subsets. Prince H.E., Weber D.M., Jensen E.R., Clin. Immunol. Immunopathol. 1991, May;58(3):419-30. _x000D_ 5. Defective clonogenic potential of CD8+ T lymphocytes in patients with AIDS. Expansion in vivo of a nonclonogenic CD3+ CD8+ DR+ CD25- T cell population. Pantaleo G., Keonig S., Baseler M., Lane H.C., Fauci A.S., J. Immunol. 1990 Mar ; 144(5):1696-704.

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4