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Mouse anti Human CD103

CAT: 0579-1031Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0579-1031Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This Human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.
CAS Number
9007-83-4
Synonyms
CD103
UniProt
P38570
Host
Mouse
Species Reactivity
Human
Isotype
IgG1
Clone
AX.14
Conjugation
Unconjugated
Type
Single-Color Reagent
Applications
Flow Cytometry
Field of Research
Flow Cytometry
Purification Method
Protein A/G Chromatography
Assay Principle
PBMC: Add 10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).
Shipping Conditions
Room Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
Flow Cytometry Staining

UniProtKB · P38570

Integrin alpha-E

ITAE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P38570
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGAE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q17RS6, Q9NZU9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

PTM

Disulfide bondGlycoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Domain

RepeatSignalTransmembraneTransmembrane helix

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