Mouse anti Human CD103
CAT:
579-1031
Size:
100 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse anti Human CD103
- Background: The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This Human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.
- Synonyms: CD103
- CAS Number: 9007-83-4
- UniProt: P38570
- Host: Mouse
- Species Reactivity: Human
- Isotype: IgG1
- Clone: AX.14
- Conjugation: Unconjugated
- Type: Single-Color Reagent
- Applications: Flow Cytometry
- Field of Research: Flow Cytometry
- Purification Method: Protein A/G Chromatography
- Assay Principle: PBMC: Add 10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
- Stability: See expiration date on vial
- Concentration: See vial for Concentration
- Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
- Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
- References & Citations: 1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).
- Shipping Conditions: Room Temperature
- Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
- Functional Analysis: Flow Cytometry Staining