Mouse anti Human CD23

Mouse anti Human CD23

• Background: CD23 antigen is an integral membrane glycoprotein, Mr. 45 kdaltons The CD23 antigen is present on approximately all pre-B lymphocytes, plasma cells CD25 positive Ki-1 cells and HLA-DR. CD23 antigen is also expressed on Lymphoma, T Cell Lymphomas and B-CLLs. CD23 antigen is present on HodgkinÕs and Reed Sternberg cells).
• CAS Number: 9007-83-4
• UniProt: P06734
• Host: Mouse
• Isotype: IgG1
• Clone: LVNC-4
• Conjugation: Unconjugated
• Type: Single-Color Reagent
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: PBMC: Add10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. CD23: a multi-lineage cell activation molecule with a split personality. Int J Clin Lab Res 1992; 22(2):73-80Malavasi F; Funaro A; Alessio M; DeMonte LB; Ausiello CM; Dianzani U; Lanza F; Magrini E; Momo M; Roggero S 2. Eleven lymphoid phenotypic markers in HIV infection: selective changes induced by zidovudine treatment. J Acquir Immune Defic Syndr 1992;5(9):890-7Bass HZ; Hardy WD; Mitsuyasu RT; Wang YX; Cumberland W; Fahey JL 3. Three-color cytofluorometric analysis of CD8 cell subsets in HIV-1 infection. J Acquir Immune Defic Syndr 1991;4(12):1227-32 Prince HE; Jensen ER
• Shipping Conditions: Room Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: Flow Cytometry Staining