Human GITR / TNFRSF18 Protein, Fc Tag

Human GITR / TNFRSF18 Protein, Fc Tag

• Background: Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
• Description: Human GITR, Fc Tag (GIR-H5254) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.0 kDa. The protein migrates as 45-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 26 - Glu 161
• Host: HEK 293
• Target: GITR
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human GITR, Fc Tag (Cat. No. GIR-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand, Avitag,Fc Tag (Cat. No. GIL-H82F8) with a linear range of 4-63 ng/mL (QC tested).
• Format: Powder
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 41.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C