GITR Antibody (TNFRSF18)

• Description:

  GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  O35714 (Mouse)

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  Mouse CD25+ CD4+ T cells were used as the immunogen for the GITR antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b λ

• Clone:

  DTA-1

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the GITR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This GITR antibody is available for research use only.

• Storage Conditions:

  Store the GITR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the GITR antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted, cell surface

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free GITR Antibody (clone DTA-1) . Confirmation of Integrity and Purity of the Antibody.