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Phospho-EIF4E Antibody (pSer209)

CAT: 0800-R20466-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20466-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated.
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:1000
UniProt
P06730
Host
Rabbit
Reactivity
Human
Immunogen
The amino acid sequence surrounding pSer209 of human EIF4E protein was used as the immunogen for the phospho-EIF4E antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM452
Type
Recombinant
Applications
WB
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant phospho-eIF4E antibody at -20oC.
Limitations
This recombinant phospho-EIF4E antibody is available for research use only.
Storage Conditions
Store the recombinant phospho-eIF4E antibody at -20°C.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of lysate of human MCF-7 cells, non-treated (-) or treated (+) with fetal bovine serum, using recombinant phospho-EIF4E antibody at a 1:1000 dilution.

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