Phospho-EIF4E Antibody (pSer209)

• Description :

  This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated.

• Specifications :

  Western blot: 1:500-1:1000

• UniProt :

  P06730

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  The amino acid sequence surrounding pSer209 of human EIF4E protein was used as the immunogen for the phospho-EIF4E antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG

• Clone :

  RM452

• Type :

  Recombinant

• Applications :

  WB

• Purity :

  Protein A purified from animal origin-free supernatant

• Format :

  Purified

• Buffer :

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution :

  Store the recombinant phospho-eIF4E antibody at -20oC.

• Limitations :

  This recombinant phospho-EIF4E antibody is available for research use only.

• Storage Conditions :

  Store the recombinant phospho-eIF4E antibody at -20°C.

• Formulation :

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9000-83-3

• Image Legend :

  Western blot testing of lysate of human MCF-7 cells, non-treated (-) or treated (+) with fetal bovine serum, using recombinant phospho-EIF4E antibody at a 1:1000 dilution.