EIF4E Antibody

• Description:

  EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt:

  P06730

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 32-61 from the human protein was used as the immunogen for this EIF4E antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This EIF4E antibody is available for research use only.

• Storage Conditions:

  Aliquot the EIF4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat, Bovine, Zebrafish, Rabbit, Xenopus

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.