EIF4E Antibody

• Description:

  EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

• Specifications:

  Western blot: 1:100-1:5000

• UniProt:

  P06730

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human EIF4E was used as the immunogen for the EIF4E antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  AFHI-5

• Type:

  Recombinant

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the EIF4E antibody at -20oC.

• Limitations:

  This EIF4E antibody is available for research use only.

• Storage Conditions:

  Store the EIF4E antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the EIF4E antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human HEK293 cell lysate with EIF4E antibody. Predicted molecular weight ~27 kDa.