EIF4E Antibody

CAT: 0800-F54054-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
CAT#:0800-F54054-0.1MLSize:0.1 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P06730
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein was used as the immunogen for this EIF4E antibody.
Clonality
Monoclonal
Isotype
LgG1
Clone
8C8-C10-C12
Applications
WB
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the EIF4E antibody at -20oC.
Limitations
This EIF4E antibody is available for research use only.
Storage Conditions
Store the EIF4E antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.

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