EIF4E Antibody

• Description :

  Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt]

• Specifications :

  Western blot: 1:1000

• UniProt :

  P06730

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A human recombinant protein was used as the immunogen for this EIF4E antibody.

• Clonality :

  Monoclonal

• Isotype :

  LgG1

• Clone :

  8C8-C10-C12

• Applications :

  WB

• Purity :

  Protein G affinity

• Format :

  Purified

• Buffer :

  Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

• Reconstitution :

  Store the EIF4E antibody at -20oC.

• Limitations :

  This EIF4E antibody is available for research use only.

• Storage Conditions :

  Store the EIF4E antibody at -20°C.

• Formulation :

  In PBS with 50% glycerol and 0.03% ProClin 300

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.