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EIF4E Antibody

CAT: 0800-F47411-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F47411-0.08MLSize:0.08 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 27-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P06730
Host
Rabbit
Reactivity
Human
Immunogen
This EIF4E antibody was produced from rabbits immunized with a recombinant protein from human VGFR2.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EIF4E antibody is available for research use only.
Storage Conditions
Aliquot the EIF4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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