EIF4E (phospho Ser209) Antibody

• Background:

  Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

• NCBI Gene ID:

  1977

• Swiss Prot:

  P06730

• Accession Number:

  NP_001124150.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  EIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  25 kDa

• Specificity:

  This antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  EIF4E

• NCBI Official Name:

  Eukaryotic translation initiation factor 4E

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461

• Other Product Names:

  CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA cap-binding protein, eIF-4F 25 kDa subunit

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  194578907

• Physical Properties:

  Liquid