Phospho-MAP2 (S136) Recombinant Rabbit Monoclonal Antibody [JE44-25]

Phospho-MAP2 (S136) Recombinant Rabbit Monoclonal Antibody [JE44-25]

• Description: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family were originally isolated since they copurify with tubulin in polymerization experiments: tubulin in cell extracts can be made to polymerize to produce microtubules (MT) under the influence of heat and the addition of GTP, and the MT can then be collected by centrifugation. When this is done a series of microtubule associated proteins are collected along with the MT and can be detected by SDS-PAGE and other methods. Brain extracts are rich in several of these proteins, MAP2 being one of these. The single MAP2 gene produces four major transcripts producing four proteins, MAP2A, MAP2B, MAP2C and MAP2D. MAP2A and MAP2B are very high molecular weight proteins, with apparent molecular weight on SDS-PAGE about 250kDa, while MAP2C and MAP2D are much lower molecular weight forms with apparent SDS-PAGE size about 70kDa. All forms of MAP2 share a common core sequence which includes MT binding domains, 18 amino acid sequences which are found in other MT associated proteins such as MAP Tau and MAP1B. The MAP2 isoforms are thought to be involved in MT assembly, which is an essential step in neuritogenesis. MAP2 serves to stabilize MT growth by crosslinking MT with intermediate filaments and other MTs. MAP2 isoforms are neuron-specific cytoskeletal proteins enriched in dendrites and perikarya, implicating a role in determining and stabilizing neuronal morphology during neuron development. As a result antibodies to MAP2 are widely used to identify neuronal cells and trace dendritic processes in experimental contexts.
• Product Name Alternative: DKFZp686I2148 antibody MAP 2 antibody MAP dendrite specific antibody MAP-2 antibody MAP2 antibody MAP2A antibody MAP2B antibody MAP2C antibody Microtubule associated protein 2 antibody Microtubule-associated protein 2 antibody MTAP2_HUMAN antibody
• Abbreviation: MAP2
• CAS Number: 9000-83-3
• Swiss Prot: P20357 Mouse, P15146 Rat
• Cellular Locus: Cytoplasm, cytoskeleton, Cell projection, dendrite.
• Host: Rabbit
• Species Reactivity: Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2.
• Isotype: IgG
• Clone: JE44-25
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IHC-Fr, IF-Tissue
• Positive Control: Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:100 ;IF-Tissue: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 200 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes