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MMP16 Antibody

CAT: 0800-R32966Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32966Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14, MMP15, and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)
UniProt
P51512
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids Y120-I296 was used as the immunogen for the MMP16 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the MMP16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MMP16 antibody is available for research use only.
Storage Conditions
After reconstitution, the MMP16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MMP16 antibody should be determined by the researcher.
Location
Cytoplasmic, membrane, secreted
Image Legend
Immunofluorescent staining of FFPE human A431 cells with MMP16 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P51512

Matrix metalloproteinase-16

MMP16_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P51512
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MMP16
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MMP-16
EC number
3.4.24.-
Processing
Precursor
Secondary accessions
B2RAN7, Q14824, Q52H48
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-bindingZinc

Cellular component

Cell membraneExtracellular matrixMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinZymogen

Biological process

Collagen degradation

Molecular function

HydrolaseMetalloproteaseProtease

Domain

RepeatSignalTransmembraneTransmembrane helix

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