EIF4B Antibody

• Description:

  EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  P23588

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 546-575 from the human protein was used as the immunogen for the EIF4B antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P, WB

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the EIF4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This EIF4B antibody is available for research use only.

• Storage Conditions:

  Aliquot the EIF4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the EIF4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human HeLa cell lysate with EIF3B antibody. Predicted molecular weight ~69 kDa.