EIF3B Antibody

• Description:

  Eukaryotic Translation Initiation Factor 3, Subunit B, also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of two proteins, p116 (EIF3B) and an unrelated 110-kD protein.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P55884

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  An amino acid sequence from the C-terminus of human Eukaryotic Translation Initiation Factor 3, Subunit B (EDFRKYRKMAQELYMEQKNER) was used as the immunogen for this Eif3b antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the EIF3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This EIF3B antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the EIF3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the EIF3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) A375, 2) K562 and 3) MCF7 cell lysate with EIF3B antibody. Expected molecular weight ~116 kDa.