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EIF3B Antibody

CAT: 0800-R31139Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R31139Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic Translation Initiation Factor 3, Subunit B, also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of two proteins, p116 (EIF3B) and an unrelated 110-kD protein.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P55884
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the C-terminus of human Eukaryotic Translation Initiation Factor 3, Subunit B (EDFRKYRKMAQELYMEQKNER) was used as the immunogen for this Eif3b antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the EIF3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This EIF3B antibody is available for research use only.
Storage Conditions
After reconstitution, the EIF3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the EIF3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) A375, 2) K562 and 3) MCF7 cell lysate with EIF3B antibody. Expected molecular weight ~116 kDa.

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