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BRAF V600E Antibody

CAT: 0800-F54150-0.05MLSize: 0.05 mLDry Ice: NoHazardous: No
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CAT#:0800-F54150-0.05MLSize:0.05 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-1:2000
UniProt
P15056
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 576-606 from human BRAF was used as the immunogen for the BRAF V600E antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the BRAF V600E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BRAF V600E antibody is available for research use only.
Storage Conditions
Aliquot the BRAF V600E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Chicken, Mouse, Rat
Image Legend
Western blot testing of 1) BRAF-600V, 2) BRAF-V600E, 3) Raji, 4) HeLa, 5) A549, 6) T47D and 7) MDA-MB-453 lysate with BRAF V600E antibody at 1:2000. Predicted molecular weight ~85-95 kDa.

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