BRAF Antibody

• Description:

  The BRAF gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. [RefSeq]

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

• UniProt:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human B Raf was used as the immunogen for the BRAF antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  CCG-2

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the BRAF antibody at -20oC.

• Limitations:

  This BRAF antibody is available for research use only.

• Storage Conditions:

  Store the BRAF antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the BRAF antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic, nuclear, cell membrane

• Image Legend:

  IHC staining of FFPE human ovarian cancer with BRAF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.