BRAF Antibody

• Description :

  The BRAF gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. [RefSeq]

• Specifications :

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

• UniProt :

  P15056

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A synthetic peptide specific to human B Raf was used as the immunogen for the BRAF antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG

• Clone :

  CCG-2

• Type :

  Recombinant

• Applications :

  IHC-P, WB

• Purity :

  Affinity purified

• Format :

  Purified

• Buffer :

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution :

  Store the BRAF antibody at -20oC.

• Limitations :

  This BRAF antibody is available for research use only.

• Storage Conditions :

  Store the BRAF antibody at -20°C.

• Formulation :

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes :

  Optimal dilution of the BRAF antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Cytoplasmic, nuclear, cell membrane

• Image Legend :

  IHC staining of FFPE human ovarian cancer with BRAF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.