BRAF Antibody

• Description :

  BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

• Specifications :

  Western blot: 1:500, IHC (Paraffin) : 1:10-1:50

• UniProt :

  P15056

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 580-609 from the human protein was used as the immunogen for this BRAF antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This BRAF antibody is available for research use only.

• Storage Conditions :

  Aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Chicken, Mouse

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of lysate from MCF-7 cell line using BRAF antibody. Predicted size 85-95 kDa