BRAF Antibody

• Description :

  BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt :

  P15056

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, IF, FACS, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Store at 4oC for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This BRAF antibody is available for research use only.

• Storage Conditions :

  Store at 4°C for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Mouse, Chicken

• Image Legend :

  Western blot analysis of BRAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B-RAF gene (2) . Predicted size 85-95 kDa