BRAF Antibody

• Description:

  BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

• Specifications:

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50

• UniProt:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This BRAF antibody was produced from rabbits immunized with B-RAF recombinant protein.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This BRAF antibody is available for research use only.

• Storage Conditions:

  Aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  BRAF antibody western blot analysis in (1) HeLa and (2) T47D lysate. Predicted size 85-95 kDa