BRAF Antibody

• Description:

  BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, IF, FACS, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Store at 4oC for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This BRAF antibody is available for research use only.

• Storage Conditions:

  Store at 4°C for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Chicken

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of BRAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B-RAF gene (2) . Predicted size 85-95 kDa