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B-Actin Antibody

CAT: 0800-F40216-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F40216-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000, Flow cytometry: 1:25
UniProt
P60709
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
137CT26.1.1
Applications
WB, IF, IHC, FACS
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the b-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This b-Actin antibody is available for research use only.
Storage Conditions
Aliquot the b-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Chicken, Pig, Xenopus
Image Legend
Western blot analysis of b-Actin antibody in 1) K562, 2) HL-60, 3) HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate

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