G-Actin Antibody / Gamma Actin / ACTG1

• Description:

  Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

• Specifications:

  Western blot: 1:1000, Flow cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  P63261

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 188-215 from human ACTG1 was used as the immunogen for this g-Actin antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, FACS, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the g-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This g-Actin antibody is available for research use only.

• Storage Conditions:

  Aliquot the g-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the g-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine, C. elegans, Chicken, Drosophila, Mouse, Rabbit, Rat, Xenopus, Zebrafish

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of g-Actin antibody and (1) K562 and (2) HepG2 lysate.