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Beta Actin Antibody

CAT: 0800-R30100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
AC-15
Applications
WB, IHC-P
Purity
Unpurified ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the beta-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This beta-Actin antibody is available for research use only.
Storage Conditions
After reconstitution, the beta-Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of beta-Actin antibody and rat tissue: ovary (1), testis (2), heart (3), brain (4) ; and human cell lines, lanes 5: A453; 6: HeLa; 7: SMMC-7721; 8: HT1080; 9: SW620 cell lysate

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