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Actin Antibody / ACTA

CAT: 0800-R30001Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30001Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
CAS Number
9007-83-4
Specifications
Western blot: 2 µg/mL, IHC (Frozen) : 4 µg/mL to detect Actin in methanol-fixed tissues
Host
Mouse
Reactivity
Human, Mouse, Rat, Chicken
Immunogen
A C-terminal peptide (SGPSIVHRKCF) from human ACTA was used as the immunogen for this Actin antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
AC-40
Applications
WB, IHC-F
Purity
Unpurified ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Actin antibody is available for research use only.
Storage Conditions
After reconstitution, the Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC-F testing of frozen human placenta with Actin antibody.

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