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Actin Antibody / ACTA1

CAT: 0800-R32165Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32165Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemsitry (FFPE) : 2-5 µg/mL
UniProt
P68133
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY of human Actin were used as the immunogen for the Actin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Actin antibody is available for research use only.
Storage Conditions
After reconstitution, the Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Actin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE mouse heart tissue with Actin antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

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