Complement C5 Recombinant Protein

CAT: 0223-11-357Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-11-357Size:0.1 mg
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Background
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain) . Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
CAS Number
9000-83-3
Tag
His Tag
Type
Recombinant Proteins
Source
HEK293 cells
Purity
>95% as determined by SDS-PAGE.
Buffer
PBS, pH7.4
Shipping Conditions
Room Temp
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
73.5 kDa (β chain) & 114.6 kDa (α chain)
Applications Notes
This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α
Background Reference 01
DeMartino JA, et al. 1994. J Biol Chem. 269 (20) :14446-50.
Background Reference 02
Fredslund F, et al. 2008. Nat Immunol. 9 (7) :753-60.
Background Reference 03
Chen Z, et al. 1998. J Biol Chem. 273 (17) :10411-9.
Other Product Names
Complement C5, C5, CPAMD4
Tested Applications
ELISA, WB
Species
Mouse
Physical Properties
Lyophilized

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