Complement Component C5a Recombinant Protein

• Background:

  Human Complement Component C5a (C5a) is also known as C5, and is a protein fragment released from complement component C5., is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

• Tag:

  Tag Free

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  8.3 kDa

• Applications Notes:

  This protein carries no "tag". The protein has a calculated MW of 8.3 kDa. The protein migrates as 9 kDa under reducing (R) condition (SDS-PAGE) .

• Background Reference 01:

  Gerard, C. and N.P. Gerard, 1994, Annu. Rev. Immunol.12:775.

• Background Reference 02:

  Monk, PN. et al., 2007, British Journal of Pharmacology. 152: 429-48.

• Background Reference 03:

  Reis ES, et al., 2011, Brain Behav Immun. Apr 23.

• Background Reference 04:

  Bergseth G, et al., 2011, Ann Thorac Surg., 91 (2) :527-33.

• Other Product Names:

  C5a, Complement Component 5a

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3