Complement Component C5a Recombinant Protein

• Background :

  Complement Component C5a (C5a) is also known as C5, and is a protein fragment released from complement component C5., is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

• NCBI Gene ID :

  102125658

• Accession Number :

  XP_015292262.1

• Tag :

  Fc Tag

• Type :

  Recombinant Proteins

• Source :

  HEK293 cells

• Purity :

  >90% as determined by SDS-PAGE.

• Buffer :

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions :

  Room Temp

• Storage Conditions :

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight :

  34.6 kDa

• Applications Notes :

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name :

  ComplementC5

• NCBI Organism :

  Macaca fascicularis

• Background Reference 01 :

  Gerard, C. and N.P. Gerard, 1994, Annu. Rev. Immunol.12:775.

• Background Reference 02 :

  Monk, PN. et al., 2007, British Journal of Pharmacology. 152: 429-48.

• Background Reference 03 :

  Reis ES, et al., 2011, Brain Behav Immun. Apr 23.

• Background Reference 04 :

  Bergseth G, et al., 2011, Ann Thorac Surg., 91 (2) :527-33.

• Other Product Names :

  C5a, Complement Component 5a

• Tested Applications :

  ELISA, WB

• Species :

  Human

• Physical Properties :

  Lyophilized

• CAS Number :

  9000-83-3