Complement Component C2 Recombinant Protein

• Background:

  C2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.

• NCBI Gene ID:

  717

• Swiss Prot:

  P06681

• Accession Number:

  NP_000054.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  20 mM Tris, 150 mM NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  83.0 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

• NCBI Official Name:

  ComplementC2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mortensen S, et al. 2016. J Biol Chem. 291 (32) :16494-507.

• Background Reference 02:

  Yaseen S, et al. 2017. FASEB J. 31 (5) :2210-2219.

• Other Product Names:

  C2, Complement C2, Complement Component C2

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3