Complement C5 Recombinant Protein

• Background:

  Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain) . Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

• Accession Number:

  XP_001079130.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  74.1 kDa (β chain) & 114.8 kDa (α chain)

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α

• Background Reference 01:

  DeMartino JA, et al. 1994. J Biol Chem. 269 (20) :14446-50.

• Background Reference 02:

  Fredslund F, et al. 2008. Nat Immunol. 9 (7) :753-60.

• Background Reference 03:

  Chen Z, et al. 1998. J Biol Chem. 273 (17) :10411-9.

• Other Product Names:

  Complement C5, C5, CPAMD4

• Tested Applications:

  ELISA, WB

• Species:

  Rat

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3