Ac-DEVD-AMC

• Description:

  Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm[1][2][3].

• UNSPSC:

  12171500

• Target:

  Fluorescent Dye

• Type:

  Dye Reagents

• Related Pathways:

  Others

• Applications:

  Cancer-programmed cell death

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/Ac-DEVD-AMC.html

• Purity:

  99.93

• Solubility:

  DMSO : 10 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL

• Smiles:

  CC (C1=CC=C (NC ([C@H] (CC (O) =O) NC ([C@H] (C (C) C) NC ([C@H] (CCC (O) =O) NC ([C@@H] (NC (C) =O) CC (O) =O) =O) =O) =O) =O) C=C1O2) =CC2=O

• Molecular Formula:

  C30H37N5O13

• Molecular Weight:

  675.64

• References & Citations:

  [1]Nobel CS, et al. Disulfiram is a potent inhibitor of proteases of the caspase family. Chem Res Toxicol. 1997 Dec;10 (12) :1319-24. |[2]Ochu EE, et al. Caspases mediate 6-hydroxydopamine-induced apoptosis but not necrosis in PC12 cells. J Neurochem. 1998 Jun;70 (6) :2637-40. |[3]Yakovlev AA, Gorokhovatsky AY, Onufriev MV, Beletsky IP, Gulyaeva NV. Brain cathepsin B cleaves a caspase substrate. Biochemistry (Mosc) . 2008 Mar;73 (3) :332-6.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -80°C, 2 years; -20°C, 1 year (Powder, sealed storage, away from moisture and light)

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  169332-61-0