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PPAR-gamma Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1482Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1482Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PPAR-gamma Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PPAR-gamma protein expression profile in cells. The kit can be used for measuring the relative amounts of PPAR-gamma in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PPAR-gamma.
Synonyms
Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3
Gene Name
PPARG
UniProt
P37231
Reactivity
Human, Mouse, Rat
Tissue Specificity
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity) .
Molecular Weight
57620 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Peroxisome proliferator-activated receptor gamma
Subcellular Location
Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. NOCT enhances its nuclear translocation.

UniProtKB · P37231

Peroxisome proliferator-activated receptor gamma

PPARG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P37231
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PPARG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PPAR-gamma
EC number
—
Processing
—
Secondary accessions
A8K3G6, B5BUA1, O00684, O00710, O14515, Q0QJH8, Q15178, Q15179, Q15180, Q15832, Q86U60, Q96J12
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorDNA-bindingReceptor

Coding sequence diversity

Alternative splicing

Biological process

Biological rhythmsTranscriptionTranscription regulation

Cellular component

CytoplasmNucleus

Disease

Diabetes mellitusDisease variantObesity

PTM

GlycoproteinIsopeptide bondPhosphoproteinUbl conjugation

Ligand

Metal-bindingZinc

Domain

Zinc-finger

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