IKK-gamma Colorimetric Cell-Based ELISA Kit

• Description:

  The IKK-gamma Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-gamma protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-gamma in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-gamma.

• Synonyms:

  NEMO; NF-kappa-B essential modifier; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IkB kinase subunit gamma; I-kappa-B kinase gamma; IKK-gamma; IKKG; IkB kinase-associated protein 1; IKKAP1; FIP-3

• Gene Name:

  IKBKG

• UniProt:

  Q9Y6K9

• Reactivity:

  Human

• Tissue Specificity:

  Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

• Molecular Weight:

  48198 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  NF-kappa-B essential modulator

• Subcellular Location:

  Cytoplasm. Nucleus. Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.