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ADD1/2 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1016Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1016Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The ADD1/2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ADD1/2 protein expression profile in cells. The kit can be used for measuring the relative amounts of ADD1/2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ADD1/2.
Synonyms
Alpha-adducin; Erythrocyte adducin subunit alpha; ADD1; ADDA
Gene Name
ADD1
UniProt
P35611
Reactivity
Human, Mouse, Rat
Tissue Specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Molecular Weight
80955 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Alpha-adducin
Subcellular Location
Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

UniProtKB · P35611

Alpha-adducin

ADDA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35611
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ADD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A2A3N8, A2A3P0, B4DI79, D3DVR3, D3DVR4, D3DVR5, Q13734, Q14729, Q16156, Q86XM2, Q9UJB6
Protein keywords

PTM

AcetylationPhosphoprotein

Molecular function

Actin-bindingCalmodulin-binding

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmCytoskeletonMembrane

Technical term

Direct protein sequencingProteomics identificationReference proteome

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