BCL-2 Colorimetric Cell-Based ELISA Kit

• Description:

  The BCL-2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BCL-2 protein expression profile in cells. The kit can be used for measuring the relative amounts of BCL-2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BCL-2.

• Synonyms:

  Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; BCL2

• Gene Name:

  BCL2

• UniProt:

  P10415

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in a variety of tissues.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .

• Molecular Weight:

  26266 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Apoptosis regulator Bcl-2

• Subcellular Location:

  Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.