Mouse anti Human CD4 FITC - CD8 PE

• Background:

  Identification of CD4 on Human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on Human T cells suppressor/cytotoxic expressing the 32,000 M.W. alpha subunit surface antigens.

• Synonyms:

  CD4 FITC - CD8 PE

• UniProt:

  P01730, P01732

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Conjugation:

  Bi-Test (FITC/RPE) Reagent

• Type:

  Bi-Testª Reagents (FITC/RPE)

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

• Stability:

  See expiration date on vial

• Concentration:

  Titered for flow cytometry

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans, R.L., Wall, D.W., Platsoucas, C.D., Siegal, F.P., Fikrig, S.M., Testa, C.M, and Good, R.A. Proc. Nat. Acad. Sci. 78,544,1981. 2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E. J. Immunology 145(1):102-8,1990 . 3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K., J. Leukoc. Biol. 47(2): 111-20,1990. 4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990. 5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C., Nat. 349(6309):533-5,1991. 7. Evolutionary Conservation of Surface Molecules that Distinguish T Lymphocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. Ledbetter, J.A., Evans, R.L., Lipinski, M., Cunningham-Rundles, C., Good, R.A., and Herzenberg, L.A., J. Exp. Med. 153,310, 1981. 8. Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Engleman, E.G., Benike, C.J., and Evans, R.L., Clin. Res. 29, 365a 1981. 9. Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. Kotzin, B.L., Benike, C.J. and Engleman, E.G., J. Imm. 127,931,1981. 10. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Gallagher, P.F., Fazekas de St. Groth, B., Miller, J.F.A.P., Proc. Nat. Acad. Sci. 1989,86:10044.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  Product should be stored at 4-8ºC; DO NOT FREEZE

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4